An Impedance Aptasensor with Microfluidic Chips for Specific Detection of H5N1 Avian Influenza Virus.
Identifieur interne : 000596 ( Main/Exploration ); précédent : 000595; suivant : 000597An Impedance Aptasensor with Microfluidic Chips for Specific Detection of H5N1 Avian Influenza Virus.
Auteurs : Jacob Lum [États-Unis] ; Ronghui Wang [États-Unis] ; Billy Hargis [États-Unis] ; Steve Tung [États-Unis] ; Walter Bottje [États-Unis] ; Huaguang Lu [États-Unis] ; Yanbin Li [États-Unis]Source :
- Sensors (Basel, Switzerland) [ 1424-8220 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Aptamères nucléotidiques (), Grippe chez les oiseaux (virologie), Impédance électrique, Microfluidique (instrumentation), Oiseaux (virologie), Sous-type H5N1 du virus de la grippe A (isolement et purification), Techniques d'analyse microfluidique (instrumentation), Techniques de biocapteur (instrumentation).
- MESH :
- isolement et purification : Sous-type H5N1 du virus de la grippe A.
- virologie : Grippe chez les oiseaux, Oiseaux.
- Animaux, Aptamères nucléotidiques, Impédance électrique, Microfluidique, Techniques d'analyse microfluidique, Techniques de biocapteur.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Aptamers, Nucleotide.
- instrumentation : Biosensing Techniques, Microfluidic Analytical Techniques, Microfluidics.
- isolation & purification : Influenza A Virus, H5N1 Subtype.
- virology : Birds, Influenza in Birds.
- Animals, Electric Impedance.
Abstract
In this research a DNA aptamer, which was selected through SELEX (systematic evolution of ligands by exponential enrichment) to be specific against the H5N1 subtype of the avian influenza virus (AIV), was used as an alternative reagent to monoclonal antibodies in an impedance biosensor utilizing a microfluidics flow cell and an interdigitated microelectrode for the specific detection of H5N1 AIV. The gold surface of the interdigitated microelectrode embedded in a microfluidics flow cell was modified using streptavidin. The biotinylated aptamer against H5N1 was then immobilized on the electrode surface using biotin-streptavidin binding. The target virus was captured on the microelectrode surface, causing an increase in impedance magnitude. The aptasensor had a detection time of 30 min with a detection limit of 0.0128 hemagglutinin units (HAU). Scanning electron microscopy confirmed the binding of the target virus onto the electrode surface. The DNA aptamer was specific to H5N1 and had no cross-reaction to other subtypes of AIV (e.g., H1N1, H2N2, H7N2). The newly developed aptasensor offers a portable, rapid, low-cost alternative to current methods with the same sensitivity and specificity.
DOI: 10.3390/s150818565
PubMed: 26230699
Affiliations:
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Le document en format XML
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Fayetteville, AR 72701, USA. rwang@uark.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701</wicri:regionArea><wicri:noRegion>AR 72701</wicri:noRegion></affiliation></author><author><name sortKey="Hargis, Billy" sort="Hargis, Billy" uniqKey="Hargis B" first="Billy" last="Hargis">Billy Hargis</name><affiliation wicri:level="1"><nlm:affiliation>Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. bhargis@uark.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701</wicri:regionArea><wicri:noRegion>AR 72701</wicri:noRegion></affiliation></author><author><name sortKey="Tung, Steve" sort="Tung, Steve" uniqKey="Tung S" first="Steve" last="Tung">Steve Tung</name><affiliation wicri:level="1"><nlm:affiliation>Department of Mechanical Engineering, University of Arkansas, Fayetteville, AR 72701, USA. chstung@uark.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Mechanical Engineering, University of Arkansas, Fayetteville, AR 72701</wicri:regionArea><wicri:noRegion>AR 72701</wicri:noRegion></affiliation></author><author><name sortKey="Bottje, Walter" sort="Bottje, Walter" uniqKey="Bottje W" first="Walter" last="Bottje">Walter Bottje</name><affiliation wicri:level="1"><nlm:affiliation>Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. wbottje@uark.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701</wicri:regionArea><wicri:noRegion>AR 72701</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Huaguang" sort="Lu, Huaguang" uniqKey="Lu H" first="Huaguang" last="Lu">Huaguang Lu</name><affiliation wicri:level="4"><nlm:affiliation>Animal Diagnostic Laboratory, Pennsylvania State University, University Park, PA 16802, USA. hxl15@psu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Animal Diagnostic Laboratory, Pennsylvania State University, University Park, PA 16802</wicri:regionArea><orgName type="university">Université d'État de Pennsylvanie</orgName><placeName><settlement type="city">University Park (Pennsylvanie)</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Li, Yanbin" sort="Li, Yanbin" uniqKey="Li Y" first="Yanbin" last="Li">Yanbin Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA. yanbinli@uark.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701</wicri:regionArea><wicri:noRegion>AR 72701</wicri:noRegion></affiliation></author></analytic><series><title level="j">Sensors (Basel, Switzerland)</title><idno type="eISSN">1424-8220</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Aptamers, Nucleotide (chemistry)</term><term>Biosensing Techniques (instrumentation)</term><term>Birds (virology)</term><term>Electric Impedance</term><term>Influenza A Virus, H5N1 Subtype (isolation & purification)</term><term>Influenza in Birds (virology)</term><term>Microfluidic Analytical Techniques (instrumentation)</term><term>Microfluidics (instrumentation)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Aptamères nucléotidiques ()</term><term>Grippe chez les oiseaux (virologie)</term><term>Impédance électrique</term><term>Microfluidique (instrumentation)</term><term>Oiseaux (virologie)</term><term>Sous-type H5N1 du virus de la grippe A (isolement et purification)</term><term>Techniques d'analyse microfluidique (instrumentation)</term><term>Techniques de biocapteur (instrumentation)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Aptamers, Nucleotide</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term><term>Microfluidic Analytical Techniques</term><term>Microfluidics</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe chez les oiseaux</term><term>Oiseaux</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Birds</term><term>Influenza in Birds</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Electric Impedance</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Aptamères nucléotidiques</term><term>Impédance électrique</term><term>Microfluidique</term><term>Techniques d'analyse microfluidique</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this research a DNA aptamer, which was selected through SELEX (systematic evolution of ligands by exponential enrichment) to be specific against the H5N1 subtype of the avian influenza virus (AIV), was used as an alternative reagent to monoclonal antibodies in an impedance biosensor utilizing a microfluidics flow cell and an interdigitated microelectrode for the specific detection of H5N1 AIV. The gold surface of the interdigitated microelectrode embedded in a microfluidics flow cell was modified using streptavidin. The biotinylated aptamer against H5N1 was then immobilized on the electrode surface using biotin-streptavidin binding. The target virus was captured on the microelectrode surface, causing an increase in impedance magnitude. The aptasensor had a detection time of 30 min with a detection limit of 0.0128 hemagglutinin units (HAU). Scanning electron microscopy confirmed the binding of the target virus onto the electrode surface. The DNA aptamer was specific to H5N1 and had no cross-reaction to other subtypes of AIV (e.g., H1N1, H2N2, H7N2). The newly developed aptasensor offers a portable, rapid, low-cost alternative to current methods with the same sensitivity and specificity. </div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>University Park (Pennsylvanie)</li></settlement><orgName><li>Université d'État de Pennsylvanie</li></orgName></list><tree><country name="États-Unis"><noRegion><name sortKey="Lum, Jacob" sort="Lum, Jacob" uniqKey="Lum J" first="Jacob" last="Lum">Jacob Lum</name></noRegion><name sortKey="Bottje, Walter" sort="Bottje, Walter" uniqKey="Bottje W" first="Walter" last="Bottje">Walter Bottje</name><name sortKey="Hargis, Billy" sort="Hargis, Billy" uniqKey="Hargis B" first="Billy" last="Hargis">Billy Hargis</name><name sortKey="Li, Yanbin" sort="Li, Yanbin" uniqKey="Li Y" first="Yanbin" last="Li">Yanbin Li</name><name sortKey="Lu, Huaguang" sort="Lu, Huaguang" uniqKey="Lu H" first="Huaguang" last="Lu">Huaguang Lu</name><name sortKey="Tung, Steve" sort="Tung, Steve" uniqKey="Tung S" first="Steve" last="Tung">Steve Tung</name><name sortKey="Wang, Ronghui" sort="Wang, Ronghui" uniqKey="Wang R" first="Ronghui" last="Wang">Ronghui Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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